Molecular karyotype diversity in the microsporidian Encephalitozoon cuniculi.
Identifieur interne : 000E35 ( Main/Exploration ); précédent : 000E34; suivant : 000E36Molecular karyotype diversity in the microsporidian Encephalitozoon cuniculi.
Auteurs : C. Biderre [France] ; A. Mathis ; P. Deplazes ; R. Weber ; G. Metenier ; C P VivaresSource :
- Parasitology [ 0031-1820 ] ; 1999.
English descriptors
- KwdEn :
- Animals, Chromosome Mapping, DNA Probes (chemistry), DNA, Protozoan (chemistry), Dogs, Electrophoresis, Gel, Pulsed-Field, Encephalitozoon cuniculi (chemistry), Encephalitozoon cuniculi (genetics), Encephalitozoonosis (prevention & control), Foxes, Genetic Variation (genetics), Humans, Mice, Microsatellite Repeats, Nucleic Acid Hybridization, Polymorphism, Genetic, Rabbits.
- MESH :
- chemical , chemistry : DNA Probes, DNA, Protozoan.
- chemistry : Encephalitozoon cuniculi.
- genetics : Encephalitozoon cuniculi, Genetic Variation.
- prevention & control : Encephalitozoonosis.
- Animals, Chromosome Mapping, Dogs, Electrophoresis, Gel, Pulsed-Field, Foxes, Humans, Mice, Microsatellite Repeats, Nucleic Acid Hybridization, Polymorphism, Genetic, Rabbits.
Abstract
The microsporidian Encephalitozoon cuniculi can infect numerous mammals, including man. Three strains of E. cuniculi have been identified so far, the major marker being the number of a tetranucleotide repeats in the rDNA internal transcribed spacer. We investigated diversity at the chromosomal level through the electrophoretic karyotypes obtained from 15 E. cuniculi isolates from 5 different host species. All preparations provided patterns with 9-12 bands within a narrow molecular size range. Six karyotype forms were distinguished, involving subdivision of strain I into 3 types (A, B, C) and strain II into 2 types (D, E). The types A, B and C were mainly associated with isolates from rabbits of different geographical origins. The types D, E and F were characterized by a reduced chromosome size range, 2 of these appearing specific to a carnivorous host species (D in dog and F in blue fox). Hybridization experiments showed that all E. cuniculi isolates possess 11 chromosomes, with a size polymorphism entailing occasional electrophoretic comigration of heterologous chromosomes and differential migration of homologous ones. DNA rearrangements should occur during mitosis and the hypothesis of diploidy for the basic state of E. cuniculi seems likely.
PubMed: 10363276
Affiliations:
- France
- Auvergne (région administrative), Auvergne-Rhône-Alpes
- Clermont-Ferrand
- Université Blaise-Pascal
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Le document en format XML
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Biderre</name><affiliation wicri:level="4"><nlm:affiliation>Laboratoire de Protistologie Moléculaire et Cellulaire des Parasites Opportunistes, Université Blaise Pascal, Aubière, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Protistologie Moléculaire et Cellulaire des Parasites Opportunistes, Université Blaise Pascal, Aubière</wicri:regionArea><wicri:noRegion>Aubière</wicri:noRegion><orgName type="university">Université Blaise-Pascal</orgName><placeName><settlement type="city">Clermont-Ferrand</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region></placeName></affiliation></author><author><name sortKey="Mathis, A" sort="Mathis, A" uniqKey="Mathis A" first="A" last="Mathis">A. Mathis</name></author><author><name sortKey="Deplazes, P" sort="Deplazes, P" uniqKey="Deplazes P" first="P" last="Deplazes">P. Deplazes</name></author><author><name sortKey="Weber, R" sort="Weber, R" uniqKey="Weber R" first="R" last="Weber">R. Weber</name></author><author><name sortKey="Metenier, G" sort="Metenier, G" uniqKey="Metenier G" first="G" last="Metenier">G. Metenier</name></author><author><name sortKey="Vivares, C P" sort="Vivares, C P" uniqKey="Vivares C" first="C P" last="Vivares">C P Vivares</name></author></analytic><series><title level="j">Parasitology</title><idno type="ISSN">0031-1820</idno><imprint><date when="1999" type="published">1999</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chromosome Mapping</term><term>DNA Probes (chemistry)</term><term>DNA, Protozoan (chemistry)</term><term>Dogs</term><term>Electrophoresis, Gel, Pulsed-Field</term><term>Encephalitozoon cuniculi (chemistry)</term><term>Encephalitozoon cuniculi (genetics)</term><term>Encephalitozoonosis (prevention & control)</term><term>Foxes</term><term>Genetic Variation (genetics)</term><term>Humans</term><term>Mice</term><term>Microsatellite Repeats</term><term>Nucleic Acid Hybridization</term><term>Polymorphism, Genetic</term><term>Rabbits</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Probes</term><term>DNA, Protozoan</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Encephalitozoon cuniculi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Encephalitozoon cuniculi</term><term>Genetic Variation</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Encephalitozoonosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chromosome Mapping</term><term>Dogs</term><term>Electrophoresis, Gel, Pulsed-Field</term><term>Foxes</term><term>Humans</term><term>Mice</term><term>Microsatellite Repeats</term><term>Nucleic Acid Hybridization</term><term>Polymorphism, Genetic</term><term>Rabbits</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microsporidian Encephalitozoon cuniculi can infect numerous mammals, including man. Three strains of E. cuniculi have been identified so far, the major marker being the number of a tetranucleotide repeats in the rDNA internal transcribed spacer. We investigated diversity at the chromosomal level through the electrophoretic karyotypes obtained from 15 E. cuniculi isolates from 5 different host species. All preparations provided patterns with 9-12 bands within a narrow molecular size range. Six karyotype forms were distinguished, involving subdivision of strain I into 3 types (A, B, C) and strain II into 2 types (D, E). The types A, B and C were mainly associated with isolates from rabbits of different geographical origins. The types D, E and F were characterized by a reduced chromosome size range, 2 of these appearing specific to a carnivorous host species (D in dog and F in blue fox). Hybridization experiments showed that all E. cuniculi isolates possess 11 chromosomes, with a size polymorphism entailing occasional electrophoretic comigration of heterologous chromosomes and differential migration of homologous ones. DNA rearrangements should occur during mitosis and the hypothesis of diploidy for the basic state of E. cuniculi seems likely.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne (région administrative)</li><li>Auvergne-Rhône-Alpes</li></region><settlement><li>Clermont-Ferrand</li></settlement><orgName><li>Université Blaise-Pascal</li></orgName></list><tree><noCountry><name sortKey="Deplazes, P" sort="Deplazes, P" uniqKey="Deplazes P" first="P" last="Deplazes">P. Deplazes</name><name sortKey="Mathis, A" sort="Mathis, A" uniqKey="Mathis A" first="A" last="Mathis">A. Mathis</name><name sortKey="Metenier, G" sort="Metenier, G" uniqKey="Metenier G" first="G" last="Metenier">G. Metenier</name><name sortKey="Vivares, C P" sort="Vivares, C P" uniqKey="Vivares C" first="C P" last="Vivares">C P Vivares</name><name sortKey="Weber, R" sort="Weber, R" uniqKey="Weber R" first="R" last="Weber">R. Weber</name></noCountry><country name="France"><region name="Auvergne-Rhône-Alpes"><name sortKey="Biderre, C" sort="Biderre, C" uniqKey="Biderre C" first="C" last="Biderre">C. Biderre</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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